Multipotent Stem Cells of the Hair Follicle by Robert M Hoffman

Author:Robert M Hoffman
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

7.Cap separated from a 1.5-ml polypropylene microfuge tube. Heat an 18 G needle and make four tiny holes, one of which must be in the center of the cap (for transplantation ) while the other three are for suturing.

8.Vinyl tape.

9.Pipette and tips: 200 μl.

3 Methods

3.1 Two-Dimensional Expansion of Mesenchymal Cells

1.Anesthetize mice and perform cervical spine dislocation for euthanasia. Dissect E17 fetal skin and adult lungs (see Note 1 ). Mince the tissues with a scalpel, add 0.2 % collagenase Type I and incubate at 37 °C for 20–30 min, depending on the type of tissue.

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